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Calu-3 人類肺腺癌細胞（胸水）

簡要描述：Calu-3 人類肺腺癌細胞（胸水）
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  • 產品型號：HTB-55
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  • 更新時間：2024-11-16
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Calu-3 人類肺腺癌細胞（胸水）

ATCC® Number:HTB-55™    

Designations:Calu-3

Depositors:J Fogh

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: lung
Disease: adenocarcinoma
Derived from metastatic site: pleural effusion

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Calu-3 人類肺腺癌細胞（胸水）

Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Tumorigenic:Yes

Antigen Expression:Blood Type A; Rh+

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 12

D16S539: 12,14

D5S818: 11

D7S820: 10,11

THO1: 6,9.3

TPOX: 8

vWA: 16,17



Cytogenetic Analysis:The stemline chromosome number is hypotriploid with the 2S component occurring at 1.4%. Approximay 20 markers were common to most S metaphases, of which i (1p), t(12;?) and t(18;?) were generally paired; t(6t?) had an HSR segment of q arm, and M7 had two secondary constrictions. Normal chromosomes 1, 13, 15 and 17 were absent, and the X was disomic. No Y chromosome was detected in the QM stained preparations.

Isoenzymes:AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1

PGM3, 1-2



Age:25 years

Gender:male

Ethnicity:Caucasian

Comments:The patient had received prior therapy with cytoxan, bleomycin and adriamycin.

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C

Subculturing:Protocol:

  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week



MLE-12 小鼠肺上皮細胞

saos-2 人成骨肉瘤細胞

MA104 非洲綠猴胚胎腎上皮細胞

ball-1 細胞

nci-h226 人肺鱗癌細胞

MCA38 小鼠結腸癌細胞

LTPA 小鼠胰腺癌細胞

NCI-H23 人非小細胞肺癌細胞

293.00     人胚腎細胞

293FT    人胚腎細胞-用于慢病毒包裝

293TN    人胚腎細胞--用于慢病毒包裝

293AV    人胚腎細胞-用于腺病毒包裝

293T    人胚腎細胞

208F    大鼠成纖維細胞

786-O    人腎透明細胞腺癌細胞

A172    人腦膠質母細胞瘤細胞

A549    人肺癌細胞 細胞系購買

AGS    人胃癌細胞

BEL7402 人肝癌細胞系

AZ-521    人胃癌細胞

B16F0    小鼠黑色素瘤細胞

Bel-7402    人肝癌細胞

Bel-7404    人肝癌細胞

BGC-823    人胃癌細胞

C6    大鼠腦膠質瘤細胞

SW480    人結腸腺癌細胞

CaCo-2    人結腸癌細胞

Cho-K1    倉鼠卵巢細胞亞株

COS-7    非洲綠猴SV40轉化的腎細胞

GES-1    人胃上皮細胞

HCT-8    人回盲腸癌細胞

HeLa

Hepa1-6    小鼠肝癌細胞

HepG2    人肝癌細胞

hFOB1.19    人永生化成骨細胞

HGC-27    人胃癌細胞

Hs 746T    人胃癌細胞

HT-29    人結腸癌細胞

KB    人口腔表皮樣癌細胞

LoVo    人結腸癌細胞

LLC-PK1    豬腎近曲小管上皮細胞

MCF-10A    人正常乳腺上皮細胞

MCF-7    人乳腺癌細胞

MDA-MB-231    人乳腺癌細胞

MGC80-3    人胃癌細胞

MKN-45    人胃癌細胞

NCI-H460    人大細胞肺癌細胞

NIH-3T3    小鼠成纖維細胞

NUGC-3    人胃癌細胞

Raw264-7    小鼠單核細胞白血病細胞

SGC-7901    人胃癌細胞

SMMC-7721    人肝癌細胞

SNU-638    人胃癌細胞

SNU-739    人肝癌細胞

Tca8113    人舌鱗癌細胞    細胞系購買

U251    人腦膠質瘤細胞

U-373MG    人腦神經膠質瘤細胞

U-87 MG    人腦星形膠質母細胞瘤

BT-474    人乳腺導管癌細胞

PANC-1    人胰腺癌細胞

HEK293    人胚腎細胞

Hep3B    人肝癌細胞

HL-7702    人正常肝細胞

Raw-Blue    小鼠單核細胞白血病細胞

WEHI-231    小鼠B淋巴細胞

OPM-2    人骨髓瘤細胞

NCI-H929    人骨髓瘤細胞

U266    人骨髓瘤細胞

EJ    人膀胱癌細胞

HOC    大鼠肝卵圓細胞

RWPE-1    人正常前列腺上皮細胞

CAL 27    人舌鱗癌細胞

Calu-3    人肺腺癌細胞

Eca-109    人食管癌細胞

DLD-1    人結直腸腺癌上皮細胞

5637.00     人膀胱癌細胞

SK-OV-3    人卵巢癌細胞

GBC-SD    人膽囊癌細胞

BEL7402 人肝癌細胞系

SW620    人結腸癌細胞

MRC-5    人胚肺成纖維細胞     細胞系購買

PC9    人肺癌細胞

PC14    人肺癌細胞

BxPC    人原位胰腺腺癌細胞

PK（15）    豬腎上皮細胞

MDCK    狗腎上皮細胞

3D4/21    豬肺泡巨噬細胞

MHCC-97L    人肝癌細胞（低轉移）

MHCC-97H    人肝癌細胞系（高轉移）

MiaCaPa-2    人胰腺癌細胞

HN13    口腔鱗狀細胞癌細胞

Bend3    小鼠腦微血管內皮細胞

QBC939    人膽管癌細胞

Ishikawa    人子宮內膜癌細胞

A498    人腎癌細胞

143B

WEHI-231    小鼠B淋巴細胞

H322    人非小細胞肺癌細胞

Panc O2

S8    BEL7402 人肝癌細胞系

R5    細胞系購買

Pig MSC    豬骨髓間質干細胞

HCAEC    人冠狀動脈內皮細胞

marc-145    猴胚胎腎上皮細胞

3D4/21    豬肺泡巨噬細胞

PK（15）    豬腎上皮細胞

MADB-106    大鼠乳腺癌細胞

Sf9    昆蟲卵巢細胞

DADY

HUVEC[HUV-EC-C]    人臍靜脈內皮細胞

PC14    人肺癌細胞

PC9    人肺癌細胞

Eca-109    人食管癌細胞

CAL 27    人舌鱗癌細胞

HOC    肝卵圓細胞

EJ    人膀胱癌細胞

U266    人骨髓瘤細胞

293-mTLR5    人胚腎細胞

Raw-Blue    小鼠單核細胞白血病細胞

HL-7702    人正常肝細胞

Hep3B    人肝癌細胞

U-373MG    人腦神經膠質瘤細胞

Tca-8113    人舌鱗癌細胞

SNU-739    人肝癌細胞

SNU-638    人胃癌細胞

NUGC-3    人胃癌細胞

NIH-3T3    小鼠成纖維細胞

MKN-45    人胃癌細胞

LLC-PK1    豬腎近曲小管上皮細胞

Hs 746T    人胃癌細胞

Hepa1-6    小鼠肝癌細胞

HCT-8    人回盲腸癌細胞

BEL7402 人肝癌細胞系

HaCaT    人永生化表皮細胞

GES-1    人胃上皮細胞

B16-F0    小鼠黑色素瘤細胞

AZ-521    人胃癌細胞

786-O    人腎透明細胞腺癌細胞

208F    大鼠成纖維細胞

293TN    人胚腎細胞--用于慢病毒包裝

293FT    人胚腎細胞-用于慢病毒包裝

GP-293    人胚腎細胞

293-L.P.    人胚腎細胞

HTB-55 Calu-3 人類肺腺癌 （胸水）
















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